Evaluation of C3H/HeJ Mice for Xenodiagnosis of Infection with Ehrlichia Chaffeensis
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چکیده
منابع مشابه
Evaluation of C3H/HeJ mice for xenodiagnosis of infection with Ehrlichia chaffeensis.
Because mice are experimentally susceptible to infection with Ehrlichia species, C3H/HeJ mice were evaluated as a potential xenodiagnostic model for detection of infection with and isolation of E. chaffeensis. Intraperitoneal inoculation of mice with E. chaffeensis-infected DH82 cell cultures produced seroconversion, with peak serum antibody titers of 1:256, at high dosages (>1.9 x 10(4) infect...
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To determine whether Ehrlichia chaffeensis exists in Japan, we used PCR to examine blood from sika deer in Nara, Japan. Of 117 deer, 36 (31%) were infected with E. chaffeensis. The E. chaffeensis 16S rRNA base and GroEL amino acid sequences from Japan were most closely related to those of E. chaffeensis Arkansas.
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Thirty-eight domestic goats from an area of Ehrlichia chaffeensis endemicity were tested for antibodies reactive to E. chaffeensis and for E. chaffeensis-specific 16S rRNA gene fragments by an indirect fluorescent antibody test and a nested PCR assay, respectively. Twenty-eight of 38 (73.7%) goats had antibodies reactive to E. chaffeensis (>/=1:128), and 6 of 38 (15.8%) goats were positive by d...
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ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 1999
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063879901100109